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This is the largest of the American tree frogs. Males generally have a maximum size of 5.1-6.3 cm (SVL), with females being much larger, attaining a size of 12.7 cm (SVL). Osteopilus septentrionalis is highly variable in color, but they are usually some shade of gray, tan or olive, occasionally with dark, marbled blotches dorsally. Their skin is very textured, and they have extremely large eyes and toe pads
Typical of tree frogs, O. septentrionalis spends most of its time in trees or very large plants, especially those with large leaves.They are found only where low temperatures stay above 10 degrees C, with daytime temperatures between 23-29 degrees. This species also prefers areas of high humidity, thus it is very common along waterways. As O. septentrionalis has been introduced, it has become particularly abundant around the pool areas of hotels, where the comination of large decorative vegetation and abundant food resources have made for a favorable habitat (Bartlett,1996).